Bradford Protein Assay Calculation

Introduction to the Bradford Protein Assay Calculation

The Bradford protein assay is a biochemical test for determining the total protein concentration within a sample. The assay is based on the absorbance shift of the Coomassie Brilliant Blue dye when it binds to proteins. In this calculation, the concentration of the protein is determined by the absorbance measured at 595 nm and a known factor derived from a standard curve, typically around 0.2 for illustration purposes here.

Parameter usage:

• absorbance = absorbance reading from the spectrophotometer at 595 nm

Example valid values:

• absorbance = 0.75

Output:

• concentration = calculated protein concentration based on standard curve

Data validation

The absorbance should be greater than zero and less than 1.5 to fall within the linear range of the Bradford assay.